Abstract

Review Article

Evolution of Antifungal Activity of Artemisia herba-alba Extracts on Growth of Aspergillus sp. and Rhizopus sp.

Eman MG Gebreil, Nagwa SA Alraaydi, Saleh HM EL-Majberi and Idress Hamad Attitalla*

Published: 11 February, 2025 | Volume 8 - Issue 1 | Pages: 001-006

Plant extracts and their constituents have a long history as antifungal agents, but their use in biotechnology as preservatives, due to the increasing resistance of fungi to fungicides, has been rarely reported in Libya. The aim of this study is to evaluate the antifungal activity of ethanol extract and water extract of the wild native plant Artemisia herba-alba against two genera of mold fungi Aspergillus sp. and Rhizopus sp. This mold fungal causes significant damage to crops in the field or during storage. In this study, a hot ethanol extract was prepared using a device Soxhlet, and water extract hot as well as a cold ethanol extract and cold-water extract aqueous extract three concentrations (25% - 50% - 75%) of plant extracts were used on the tested fungi. All extracts showed an effect on the tested fungi. The concentrations of (75% - 50%) of the extracts had an effect on the tested fungi, while most concentrations of 25% of the extracts did not record any effect on the tested fungi. The hot ethanol extract of the Artemisia herba-alba plant was more effective than the other extracts. Aspergillus sp. was recorded with the highest inhibitory zone (0.73 mm).  

Read Full Article HTML DOI: 10.29328/journal.ijcmbt.1001030 Cite this Article Read Full Article PDF

Keywords:

Antifungal activity; Artemisia herba-alba; Aspergillus sp.; Rhizopus sp.

References

  1. Marzouk B, Marzouk Z, Décor R, Edziri H, Haloui E, Fenina N, et al. Antibacterial and anticandidal screening of Tunisian Citrullus colocynthis Schrad. from Medenine. J Ethnopharmacol. 2009;125(2):344-349. Available from: https://doi.org/10.1016/j.jep.2009.04.025
  2. Liu CH, Zou WX, Lu H, Tan RX. Antifungal activity of Artemisia annua endophyte cultures against phytopathogenic fungi. J Biotechnol. 2001;88(3):277-282. Available from: https://doi.org/10.1016/s0168-1656(01)00285-1
  3. Salhi N, Rahmani B, Mehani M, Terzi V, Benouaar M, Amraoui K, et al. The antifungal activity of Artemisia herba-alba aqueous extract and essential oil against storage fungus Alternaria spp and Fusarium spp. J Appl Biol Sci. 2019;13(2):108-112. Available from: https://www.jabsonline.org/index.php/jabs/article/view/606
  4. Askary RAA, Malih HR. Effectiveness study of Artemisia herba-alba and Borage officinalis leaf extract against bacteria Staphylococcus aureus. Univ Thi-Qar J Sci. 2021;8(1):113-117. Available from: https://www.iraqoaj.net/iasj/download/b6f9a55a3159a58f
  5. Ayad N, Benaraba R, Hemida H, Abdellah F. Biological activities of phenolic extracts from Artemisia herba-alba Asso grown in western Algeria. Eur J Biol Res. 2022;12(1):46-61. Available from: https://www.journals.tmkarpinski.com/index.php/ejbr/article/view/539
  6. Boukhennoufa A, Benmaghnia S, Maizi Y, Touil AMT, Meddah B. Antifungal and antioxidant activities of Artemisia herba-alba Asso. Eur J Biol Res. 2021;11(4):493-500. Available from: https://www.journals.tmkarpinski.com/index.php/ejbr/article/view/480
  7. Bourhia M, Bouothmany K, Bakrim H, Hadrach S, Salamatullah AM, Alzahrani A, et al. Chemical profiling, antioxidant, antiproliferative, and antibacterial potentials of chemically characterized extract of Citrullus colocynthis L. seeds. Separations. 2021;8(8):114. Available from: https://doi.org/10.3390/separations8080114
  8. Benmeziane S, Haddadin MS, Al-Domi HA. Extraction yield, phytochemicals analysis, and certain in vitro biological activities of Artemisia herba alba extracts. Jordan J Agric Sci. 2023;19(2):125-141. Available from: http://dx.doi.org/10.35516/jjas.v19i2.123
  9. O'Brien RM. Fungal spore concentrations in experimental treatments. Fungal Biol Rev. 2014;30(4):227-239.
  10. Vaz AB, Mota RC, Bomfim MRQ, Vieira ML, Zani CL, Rosa CA, et al. Antimicrobial activity of endophytic fungi associated with Orchidaceae in Brazil. Can J Microbiol. 2009;55(12):1381-1391. Available from: https://doi.org/10.1139/w09-101
  11. Samson RA, Varga J. Key Genera of Aspergillus and Penicillium. Stud Mycol. 2009;64:1-73.
  12. Zheng RY, Chen GQ, Huang H, Liu XY. A monograph of Rhizopus. SYDOWIA-HORN. 2007;59(2):273-372. Available from: https://www.researchgate.net/publication/287477426_A_monograph_of_Rhizopus
  13. Hrytsyk RA, Kutsyk RV, Yurchyshyn OI, Struk OA, Kireev IV, Grytsyk AR. The investigation of antimicrobial and antifungal activity of some Artemisia L. species. Pharmacia. 2021;68:93-100. Available from: https://doi.org/10.3897/pharmacia.68.e47521
  14. Baldawy MSM, Khaeim HM, Matloob AAAH. Antifungal inhibitory activity of Thymus vulgaris L. and Artemisia herba alba powder and its constituent phytochemicals against Aspergillus ochraceus and Fusarium graminearum growth. Plant Arch. 2019;19(1):801-804. Available from: https://doi.org/10.3897/pharmacia.68.e47521
  15. Zhao K, Penttinen P, Guan T, Xiao J, Chen Q, Xu J, et al. The diversity and antimicrobial activity of endophytic actinomycetes isolated from medicinal plants in Panxi plateau, China. Curr Microbiol. 2011;62:182-190. Available from: https://doi.org/10.1007/s00284-010-9685-3 

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