Abstract

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Bacteriolysis Destruction for Cu- and Zn-Ions Induced Peptidoglycan Cell Wall Based on the Halo-test Results in Cu(NO3)2 and ZnSO4 Solutions against Staphylococcus epidermidis

Tsuneo Ishida* and Reiko Kobayashi

Published: 24 March, 2026 | Volume 9 - Issue 1 | Pages: 010-017

Bacteriolysis of peptidoglycan (PGN) cell wall destruction on cleavage, hydrolysis, and decomposition by copper nitrate and zinc sulfide solutions has been investigated on the ground of the results obtained from halo antibacterial susceptibility tests in metal sulfate solutions against Staphylococcus epidermidis, in which from halo-antibacterial susceptibility tests of metallic ion concentration of 100 mM/L against Staphylococcus epidermidis, the order of bacterial effect for the metal nitrate solutions is as follows, Cu2+>Zn2+ >Ag+>Pb2+>Al3+, and the other, in the metal sulfate solutions, the antibacterial effect order is found to be Zn2+>Cu2+>Ag+>Al3+. 
Cu(Ⅱ)-, Zn(Ⅱ)-ions induced bacteriolytic destructions for PGN cell wall on cleavage, hydrolysis, and decomposition within Cu(NO3)2 and ZnSO4 solutions have been elucidated that Cu(Ⅱ) and Zn(Ⅱ) ions can inhibit PGN biosynthesis Transpeptidase (TP)/Trans-glycosylase (TG) and PGN elongation, PGN cleavage occurs by copper ions- and zinc ions-containing autolysins amidase; AmiE, Rv3717, AmiA, PGN cell wall is hydrolyzed by copper-, and zinc-containing enzymes; PGRPs, MurA, LytA, LytM=Endopeptidase, Hydrolase LytB, and PGN decomposition by copper-, zinc-containing PGN inhibitive elongation occurs. Thus, Cu(Ⅱ) and Zn(Ⅱ) within Cu(NO3)2 and ZnSO4 solutions inhibit PGN biosynthesis and activate bacterial PGN autolysins against Staphylococcus epidermidis.

Read Full Article HTML DOI: 10.29328/journal.ijcmbt.1001034 Cite this Article Read Full Article PDF

Keywords:

Zinc(Ⅱ) and copper(Ⅱ); Halo-test; Copper nitrate and zinc sulfate; PGN biosynthesis; Autolysin and elongation; S. aureus PGN cleavage; Hydrolysis and decomposition

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