Research Article

Development of ELISA based detection system against C. botulinum type B

Arti Sharma* and S Ponmariappan

Published: 30 March, 2020 | Volume 3 - Issue 1 | Pages: 017-020

Botulism is the disease caused by botulinum neurotoxins. It is produced by an obligate anaerobic bacteria called Clostridium botulinum. There is no immuno-detection system available in the world for the detection of C. botulinum. Secretory proteins of cooked meat media grown C. botulinum type B were extracted by TCA precipitation method. Polyclonal antibodies were generated against secretory proteins. Cytokine profiling of secretory proteins were done. An immunodetection system was developed to detect the C. botulinum type B using Secretory proteins of C. botulinum type B.

Read Full Article HTML DOI: 10.29328/journal.ijcmbt.1001010 Cite this Article Read Full Article PDF


Anaerobic; Botulism; ELISA; Centrifugation; Antiserum


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